Microimmunoassay using a protein chip: optimizing conditions for protein immobilization.
نویسنده
چکیده
Optimizing conditions for the microarraying of protein antigens onto glass slides were studied. Various vendors, surface functional groups, buffers, and fixatives were evaluated to enhance protein binding. A total of 125 pg of human immunoglobulin was detectable with this assay system, suggesting that protein microarray can be applied for routine immunodiagnosis.
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عنوان ژورنال:
- Clinical and diagnostic laboratory immunology
دوره 9 4 شماره
صفحات -
تاریخ انتشار 2002